Insert details here.
- Sea creatures (single appearance)(no lines)
- Hadji Singh (mentioned)
- Race Bannon (mentioned)
- McCollum (single appearance)
- Gimeno (only appearance; dies)
- Mr. Dal Chele (only appearance; dies)
- Pacific Ocean
- Chile (mentioned)
|Quinton Flynn||Jonny Quest|
Mr. Dal Chele
|Jennifer Hale||Jessie Bannon|
|John de Lancie||Dr. Benton Quest|
|Frank Welker||Sea creatures|
|Peter Jurasik||Alan Zegler|
|Karen Ross||Dr. Simone Duval|
- Hadji, Race, and Bandit are absent.
- Jonny talks about being 20,000 leagues under the sea, which is a reference to the novel Twenty Thousand Leagues Under the Sea, by Jules Verne, who is also alluded to by the naming of the Verne Research Center.
- Alan Zegler and Simone Duval's full names are revealed in the credits.
- B.J. Ward is uncredited as Iris in the Quest Byte.
- When Dr. Quest mentions his concern for the kids separately, he mentions Jessie before Jonny.
- The Real Adventures of Jonny Quest: The Complete Second Season DVD set released on November 10, 2015 by Warner Archive Collection.